This thesis interacts with the cultural values and societal expectations behind Japan’s overtime work ethic, and attempts to examine whether a universal human rights perspective can interact with the deeply rooted Japanese cultures and values. This is done with the help of previously conducted research and semi-structured interviews, which then allows for a better understanding of why overtime work is deeply rooted within Japanese culture, and why the application of human rights due diligence at the workplace is difficult to obtain. A qualitative study of semi-structured interviews has been conducted with 12 ethnic Japanese participants. The findings showed several connections between the participant’s answers, such as overtime work being an unwritten rule, and an expectation as a service to the society, to the previously conducted research. Although overtime work may change and differ in ratio depending on which company you are working for, it still is prevalent within Japanese society as a norm.Whether this is a human rights concern was debatable, since some participants argued it to be a vital part of the Japanese commitment to society, while other participants believed that it becomes problematic when personal choice and opinions are shunned or overlooked. These in turn result in other consequences such as depression, isolation, and even death. The thesis concludes that universal human rights values may not always go hand in hand with cultural values, but may allow new light to be shed on a contested phenomenon.